Result files

The pipeline generates many files, but only specific ones are defined as final outputs. These are configured in config/output_files_FFPE.yaml and config/output_files_ctDNA.yaml. {sample} is replaced with the sample name and {type} is replaced with the sample type (T/N/R, where T=Tumor, N=Normal, R=RNA).

FFPE Pipeline Output

Files defined in output_files_FFPE.yaml.

DNA

Alignment

File Description
bam_dna/{sample}_{type}.bam BAM
bam_dna/{sample}_{type}.bam.bai BAM index
bam_dna/mutect2_indel_bam/{sample}_{type}.bam Mutect2 INDEL BAM
bam_dna/mutect2_indel_bam/{sample}_{type}.bam.bai Mutect2 INDEL BAM index

SNV and INDELs

File Description
gvcf_dna/{sample}_{type}.mosdepth.g.vcf.gz Mosdepth GVCF
results/dna/{sample}_{type}/additional_files/vcf/{sample}_{type}.annotated.vcf.gz Vep annotated VCF
results/dna/{sample}_{type}/additional_files/vcf/{sample}_{type}.annotated.exon_only.filter.soft_filter.vcf Exon-only soft-filtered VCF
results/dna/{sample}_{type}/additional_files/vcf/{sample}_{type}.annotated.exon_only.filter.hard_filter.vcf Exon-only hard-filtered VCF
results/dna/{sample}_{type}/vcf/{sample}_{type}.annotated.exon_only.filter.hard_filter.codon_snv.vcf Codon SNV VCF
results/dna/{sample}_{type}/vcf/{sample}_{type}.annotated.exon_only.filter.hard_filter.codon_snv.qci.vcf QCI VCF
results/dna/{sample}_{type}/additional_files/vcf/{caller}_{sample}_{type}.vcf.gz Caller VCF
results/dna/{sample}_{type}/id_snps/{sample}_{type}.id_snps.vcf ID-SNP VCF DNA

QC

File Description
results/dna/{sample}_{type}/additional_files/qc/{sample}_{type}.duplication_metrics.txt Duplication metrics
results/dna/{sample}_{type}/additional_files/qc/{sample}_{type}.alignment_summary_metrics.txt Alignment summary metrics
results/dna/{sample}_{type}/additional_files/qc/{sample}_{type}.HsMetrics.txt HsMetrics
results/dna/{sample}_{type}/additional_files/qc/{sample}_{type}.insert_size_metrics.txt Insert size metrics
results/dna/{sample}_{type}/additional_files/qc/{sample}_{type}.samtools-stats.txt Samtools stats
results/dna/{sample}_{type}/qc/{sample}_{type}.coverage_and_mutations.tsv Coverage and mutations all
results/dna/{sample}_{type}/qc/{sample}_{type}.coverage_and_mutations.ENC.tsv Coverage and mutations ENC
results/dna/{sample}_{type}/qc/{sample}_{type}.coverage_and_mutations.URO.tsv Coverage and mutations URO
results/dna/{sample}_{type}/qc/{sample}_{type}.coverage_and_mutations.BRC.tsv Coverage and mutations BRC
results/dna/{sample}_{type}/qc/{sample}_{type}.coverage_and_mutations.CRC.tsv Coverage and mutations CRC
results/dna/{sample}_{type}/qc/{sample}_{type}.coverage_and_mutations.GIS.tsv Coverage and mutations GIS
results/dna/{sample}_{type}/qc/{sample}_{type}.coverage_and_mutations.LUN.tsv Coverage and mutations LUN
results/dna/{sample}_{type}/qc/{sample}_{type}.coverage_and_mutations.MEL.tsv Coverage and mutations MEL
results/dna/{sample}_{type}/qc/{sample}_{type}.coverage_and_mutations.TYR.tsv Coverage and mutations TYR
results/dna/{sample}_{type}/additional_files/qc/{sample}_{type}.contamination.table Contamination table
results/dna/qc/multiqc_DNA.html MultiQC DNA HTML

Reports

File Description
results/dna/{sample}_{type}/{sample}_{type}.general_report.html Results report DNA HTML
results/dna/{sample}_{type}/{sample}_{type}.prio.general_report.html Results report small panel DNA HTML

Biomarker

File Description
results/dna/{sample}_{type}/biomarker/{sample}_{type}.msisensor_pro.filtered.score.tsv MSI Sensor Pro filtered TSV
results/dna/{sample}_{type}/biomarker/{sample}_{type}.msisensor_pro.unfiltered.score.tsv MSI Sensor Pro unfiltered TSV
results/dna/{sample}_{type}/biomarker/{sample}_{type}.TMB.txt TMB
results/dna/{sample}_{type}/additional_files/biomarker/{sample}_{type}.purecn.scarhrd_cnvkit_score.txt HRD score - PureCN
results/dna/{sample}_{type}/additional_files/biomarker/{sample}_{type}.pathology.scarhrd_cnvkit_score.txt HRD score - pathology
results/dna/{sample}_{type}/biomarker/{sample}_{type}.pathology_purecn.scarhrd_cnvkit_score.txt HRD score - pathology_purecn
results/dna/{sample}_{type}/biomarker/{sample}_{type}.pathology_purecn.predicted_gis.txt Predicted GIS score

Fusions

File Description
results/dna/{sample}_{type}/fusion/{sample}_{type}.fuseq_wes.report.csv fuseq WES
results/dna/{sample}_{type}/additional_files/fusion/{sample}_{type}.fuseq_wes.unfiltered.results.csv fuseq WES unfiltered
results/dna/{sample}_{type}/fusion/{sample}_{type}.juli.filtered.fusions.txt JuLI fusions
results/dna/{sample}_{type}/additional_files/fusion/{sample}_{type}.juli.fusions.txt JuLI unfiltered fusions

CNV

File Description
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.cnvkit.scatter.png CNVkit scatter PNG
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.cnvkit.diagram.pdf CNVkit diagram PDF
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.purecn.svdb_query.vcf SVDB query - PureCN
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.pathology.svdb_query.vcf SVDB query - pathology
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.pathology_purecn.svdb_query.vcf SVDB query - pathology_purecn
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.purecn.cnv.html CNV HTML report - PureCN
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.pathology.cnv.html CNV HTML report - pathology
results/dna/{sample}_{type}/cnv/{sample}_{type}.pathology_purecn.cnv.html CNV HTML report - pathology_purecn
results/dna/{sample}_{type}/cnv/{sample}_{type}.purecn_purity_ploidity.csv PureCN purity
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.purecn.amp_all_del_all.cnv_report.tsv CNV TSV report - PureCN
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.pathology.amp_all_del_all.cnv_report.tsv CNV TSV report - pathology
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.pathology_purecn.amp_all_del_all.cnv_report.tsv CNV TSV report - pathology_purecn
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.purecn.amp_all_del_validated.cnv_report.tsv CNV TSV report validated - PureCN
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.pathology.amp_all_del_validated.cnv_report.tsv CNV TSV report validated - pathology
results/dna/{sample}_{type}/cnv/{sample}_{type}.pathology_purecn.amp_all_del_validated.cnv_report.tsv CNV TSV report validated - pathology_purecn
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.deletions.tsv Small CNV deletions
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.amplifications.tsv Small CNV amplifications
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.pathology.jumble.vcf Jumble vcf pathology
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.purecn.jumble.vcf Jumble vcf purecn
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.pathology_purecn.jumble.vcf Jumble vcf pathology_purecn
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.germline.vcf.gz Germline vcf used in CNV analysis
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.manta_tumorSV.vcf.gz Manta VCF
results/dna/{sample}_{type}/cnv/{sample}_{type}.ctDNA_fraction.tsv ctDNA fraction
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.ctDNA_fraction_info.tsv ctDNA fraction_info
results/dna/{sample}_{type}/cnv/{sample}_{type}.ITD.hard_filter.vcf ITD filtered

RNA

Alignment

File Description
bam_rna/{sample}_{type}.star_fusion.bam STAR Fusion BAM
bam_rna/{sample}_{type}.star_fusion.bam.bai STAR Fusion BAM index

SNV and INDELs

File Description
results/rna/{sample}_{type}/id_snps/{sample}_{type}.id_snps.vcf ID-SNP VCF RNA

QC

File Description
results/rna/{sample}_{type}/qc/{sample}_{type}.house_keeping_gene_coverage.tsv Housekeeping gene coverage
results/rna/qc/multiqc_RNA.html MultiQC RNA HTML
results/sample_mixup_check.tsv RNA DNA sample mixup report

Fusions

File Description
results/rna/{sample}_{type}/fusion/{sample}_{type}.fusion_report.tsv Fusion report TSV
results/rna/{sample}_{type}/additional_files/fusion/{sample}_{type}.arriba.fusions.tsv Arriba fusion TSV
results/rna/{sample}_{type}/fusion/{sample}_{type}.arriba.fusions.pdf Arriba fusion PDF
results/rna/{sample}_{type}/fusion/{sample}_{type}.exon_skipping.tsv Exon skipping TSV
results/rna/{sample}_{type}/additional_files/fusion/{sample}_{type}.ctat.exon_skipping.unfiltered.tsv CTAT-splicing unfiltered TSV
results/rna/{sample}_{type}/fusion/{sample}_{type}.ctat.exon_skipping.filtered.tsv CTAT-splicing filtered TSV
results/rna/{sample}_{type}/fusion/{sample}_{type}.ctat.exon_skipping.igv.html CTAT-splicing html
results/rna/{sample}_{type}/additional_files/fusion/{sample}_{type}.star-fusion.fusion_predictions.tsv STAR Fusion prediction TSV
results/rna/{sample}_{type}/additional_files/fusion/{sample}_{type}.fusioncatcher.fusion_predictions.txt Fusioncatcher prediction TSV

ctDNA Pipeline Output

Files defined in output_files_ctDNA.yaml that differ or are specific to ctDNA.

Alignment

File Description
bam_dna/{sample}_{type}.umi.bam BAM umi
bam_dna/{sample}_{type}.umi.bam.bai BAM umi index

SNV and INDELs

File Description
results/dna/{sample}_{type}/additional_files/vcf/{sample}_{type}.annotated.exon_only.filter.soft_filter.umi.vcf Exon-only soft-filtered VCF UMI
results/dna/{sample}_{type}/vcf/{sample}_{type}.annotated.exon_only.filter.hard_filter.codon_snv.umi.qci.vcf QCI umi VCF

Reports

File Description
results/dna/{sample}_{type}/{sample}_{type}.umi.general_report.html Results report umi DNA HTML

Biomarker

File Description
results/dna/{sample}_{type}/biomarker/{sample}_{type}.umi.TMB.txt TMB umi
results/dna/{sample}_{type}/hla/{sample}_{type}_hla_type_result.tsv HLA optitype
results/dna/{sample}_{type}/hla/{sample}_{type}_hla_type_coverage_plot.pdf HLA coverage optitype

Fusions

File Description
results/dna/{sample}_{type}/fusion/{sample}_{type}.gene_fuse_report.umi.tsv GeneFuse report
results/dna/{sample}_{type}/additional_files/fusion/{sample}_{type}.gene_fuse.unfiltered.results.txt GeneFuse fusions unfiltered

CNV

File Description
results/dna/{sample}_{type}/additional_files/cnv/{sample}_{type}.ITD.vcf ITD

Fragmentomics

File Description
results/dna/{sample}_{type}/fragmentomics/{sample}_{type}.end-motifs.tsv Fragmentomics end-motifs
results/dna/{sample}_{type}/fragmentomics/{sample}_{type}.mds.txt Fragmentomics mds
results/dna/{sample}_{type}/fragmentomics/{sample}_{type}.interval-end-motifs.tsv Fragmentomics interval-end-motifs
results/dna/{sample}_{type}/fragmentomics/{sample}_{type}.interval-mds.txt Fragmentomics interval-mds
results/dna/{sample}_{type}/fragmentomics/{sample}_{type}.frag-length-bins.tsv Fragmentomics fragment length bins
results/dna/{sample}_{type}/fragmentomics/{sample}_{type}.fragment_length_score.txt Fragmentomics fragment length score